Inadequate sample-preparation technique as a source of error in determination of erythrocyte folate by competitive binding radioassay.
نویسندگان
چکیده
منابع مشابه
The determination of erythrocyte folate concentration using a two-phase ligand-binding radioassay.
The concentration of folate in erythrocytes was determined using a two-phase ligandbIndIng radloasuy procedure described previously for measuring serum folate. The mean (± SD) folate concentration In erythrocytes of 20 normal subjects was 210 * 67 ng/mI. In 12 patients clinically folate deficient who had normal serum B2 concentration, the mean (± SD) erythrocyte folate was 71 * 39 ng/ml. Incuba...
متن کاملDetergent activation of the binding protein in the folate radioassay.
A minor cow's whey protein associated with beta-lactoglobulin is used as binding protein in the competitive radioassay for serum and erythrocyte folate. Seeking to optimize the assay, we tested the performance of binder solutions of increasing purity. The folate binding protein was isolated from cow's whey by means of CM-Sepharose CL-6B cation-exchange chromatography, and further purified on a ...
متن کاملThe Determination of Erythrocyte Folate Concentration Using a Two - Phase Ligand - binding
The concentration of folate in erythrocytes was determined using a two-phase ligandbIndIng radloasuy procedure described previously for measuring serum folate. The mean (± SD) folate concentration In erythrocytes of 20 normal subjects was 210 * 67 ng/mI. In 12 patients clinically folate deficient who had normal serum B2 concentration, the mean (± SD) erythrocyte folate was 71 * 39 ng/ml. Incuba...
متن کاملAnalytical evaluation of a folate radioassay: application to serum and erythrocyte measurements.
Many commercial folate radioassay kits have proven to be unsatisfactory in the past. It has been found that the Schwarz/Mann Iodine-125 folate radioassay kit permits a rapid measurement of folic acid requiring only a single incubation at pH 9.3. At this pH, the relative affinity of pteroylglutamic acid (FA) and N-5-methyltetrahydrofolic acid (MTFA) for the milk binder is the same. Additionally,...
متن کاملResults with commercial radioassay kits compared with microbiological assay of folate in serum and whole-blood.
We compared results with three commercial folate radioassay kits [Bio-Rad, New England Nuclear (NEN), and RIA Products] with those by microbiological assay for more than 200 samples of human serum and whole blood. All but one kit (NEN) compared favorably with the microbiological assay for serum samples, although there were notable diagnostic discrepancies. Two kits (NEN and Bio-Rad) were tested...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 23 8 شماره
صفحات -
تاریخ انتشار 1977